The study investigated the readiness of pre-service Computer Science Education Teachers for Using Open Source Applications in Universities in North East Nigeria. The study was guided by five specific objectives which culminated to five research questions. Relevant conceptual and empirical literatures were reviewed. Survey research design was adopted for the study. The population of the study comprised of 258 Pre-Service Computer Science Teachers in three universities offering Computer Science Education in North East Nigeria. Pre-Service Computer Science Education Teachers Readiness in Using Open Source Applications Questionnaire was used as an instrument for data collection.The internal consistency of the instrument was determined using Cronbach alpha and reliability coefficient of 0. 928 was obtained. Data was collected with the help of trained research assistants who assisted in the administration of questionnaires. Data collected were coded and analyzed using descriptive statistics (i.e., mean and standard deviation). The findings revealed that the Pre-service Computer Science Education Teachers in Universities in North East Nigeria are aware of Open Source Application and have adequate knowledge of Open Source Application for educational purposes. The study recommended that; government and other agencies should support and donate electronic smart boards, tablets and other ICT teaching facilities to the universities. Annual training should also be organized to increase Pre-Service Computer Science Education Teachers awareness of the available of OSA and also to equip them with the necessary skill and technical know-how on their use.